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 THE SCIENCE

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Electron micrograph of the bristles of a new toothbrush with no bacterial growth or plaque debris.

Electron micrograph of the bristles of a new toothbrush with no bacterial growth or plaque debris. Credit: STEVE GSCHMEISSNER / SCIENCE PHOTO LIBRARY

A NEW, CLEAN TOOTHBRUSH IS ESSENTIAL EVERY TIME YOU BRUSH

“The most commonly known dental plaque management method is brushing the teeth, and this is a general method of maintaining oral health and of preventing dental diseases. Brushing the teeth removes the dental plaque, food residue, and stain attached to the tooth surface, and adequately massages the periodontal tissue. Hence, it is the most important, basic, and effective method of preventing dental diseases, and of maintaining oral health [11,12].”

a significant association between dental caries infection of the tooth surface and bacterial contamination of the toothbrush has been reported .[12,13] Therefore, proper toothbrush management is needed to manage dental plaque by brushing the teeth [12,14].”

 
 

 
 

CONTAMINATION OF YOUR TOOTHBRUSH OCCURS AFTER ONE USE

Toothbrushes play an essential role in oral hygiene and are commonly found in both community and hospital settings [10]. Toothbrushes may play a significant role in disease transmission and increase the risk of infection since they can serve as a reservoir for microorganisms in healthy, oral-diseased and medically ill adults [1]. Contamination is the retention and survival of infectious organisms that occur on animate or inanimate objects. In healthy adults, contamination of toothbrushes occurs early after initial use and increases with repeated use [2, 3]. Toothbrushes can become contaminated from the oral cavity, environment, hands, aerosol contamination, and storage containers. Bacteria which attach to, accumulate, and survive on toothbrushes may be transmitted to the individual causing disease [4, 5].

Electron micrograph of plaque (green/blue) clinging to the bristle of a toothbrush after use.

Used toothbrush bristles. Coloured scanning electron micrograph (SEM) of dental plaque (green/blue) on the bristles(yellow/orange) of a used toothbrush. Plaque consists of a film of bacteria embedded in a glycoprotein matrix. The matrix is formed from bacterial secretions and saliva. Plaque is the main cause of tooth decay. The bacteria feed on sugars in food, producing acid as a waste product. This acid corrodes the teeth's enamel coating, resulting in dental caries. A build-up of dental plaque can also lead to inflamed and infected gums. Severe gum disease can lead to teeth falling out. Magnification: x750 when printed at 10 centimetres wide.Credit: STEVE GSCHMEISSNER / SCIENCE PHOTO LIBRARY

 
 

 
 

Plaque-forming bacteria, coloured scanning electron micrograph (SEM). Dental plaque is a biofilm of bacteria that forms on teeth. It can lead to tooth decay if not removed by regular brushing. These bacteria are attached to the enamel of a child's milk tooth. Magnification: x8000 when printed at 10 centimetres across. Credit: STEVE GSCHMEISSNER / SCIENCE PHOTO LIBRARY

YOUR TOOTHBRUSH CONTAINS POTENTIALLY PATHOGENIC BACTERIA AND VIRUSES

All of the studies examined toothbrush contamination and found significant bacterial retention and survival on toothbrushes after use [6, 7, 10]. Glass found that toothbrushes from both healthy patients and patients with oral disease contained potentially pathogenic bacteria and viruses such as Staphylococcus aureus, E. coli, Pseudomonas, and herpes simplex virus [1 ,10]. Glass also found toothbrushes contaminated with herpes simplex virus 1 in numbers sufficient to cause an infection in the patient [1 ,10].

 

BrushPak™…THE SCIENCE

All recommendations by BrushPak™ Affiliated Dentists are made based on Scientific Studies

 
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YOUR TOOTHBRUSH BECOMES MORE
CONTAMINATED WITH ONGOING USE

Bunetel et al. found that toothbrushes used by patients with existing oral disease quickly became contaminated [8]. This study also found a significant relationship between repeated use and bacterial retention on toothbrushes and that the oral cavity can be inoculated from a contaminated toothbrush. Caudry et al. found that toothbrushes are heavily contaminated with normal use [5]. Mehta et al. found that 70% of the toothbrushes in their study became heavily contaminated with pathogenic microorganisms after use [9, 10].

Used bristles with dental plaque (green), bacteria (blue) and cheek cells (orange) on bristle fibers (red), coloured scanning electron micrograph (SEM). Plaque consists of a biofilm of bacteria embedded in a glycoprotein matrix. The matrix is formed from bacterial secretions and saliva. The microorganisms that form the plaque biofilm are almost entirely bacteria (mainly Streptococcus mutans and other anaerobes) with the composition varying by location in the mouth. Plaque is the main cause of tooth decay. The bacteria feed on sugars in food, producing acid (lactic acid) as a waste product. This acid corrodes the teeth's enamel coating, resulting in dental cavities. A build-up of dental plaque can also lead to inflamed and infected gums. Severe gum disease can lead to periodontal disease and teeth falling out. Magnification: x70 when shortest axis printed at 25 millimetres. Credit: DENNIS KUNKEL MICROSCOPY / SCIENCE PHOTO LIBRARY

 
 

 
 
Interdental Cleaning Device (Before Use).

Interdental toothbrush shown before use with no bacterial growth or plaque, coloured scanning electron micrograph (SEM). This is a specialised toothbrush with finely separated bristles, and is used for cleaning between individual teeth. Magnification: x15 when printed at 10 centimetres across. Credit: STEVE GSCHMEISSNER / SCIENCE PHOTO LIBRARY

TOOTHBRUSHES USED REPEATEDLY OVER TIME HAVE MILLIONS TO BILLIONS OF BACTERIA

Jeong et al. reported that the toothbrushes used at home have millions to billions of bacteria, which contaminate the oral cavity when used in brushing the teeth [12, 14]. Thus, people brush their teeth every day for dental health, but they are not aware of the bacteria remaining on their toothbrushes.

Glass et al. emphasized that infection due to a contaminated toothbrush can be a risk factor not only for dental health but also for the general health [11, 12], and Caudry et al. reported that toothbrush contamination increases the dental caries [5]. 

Toothbrushes can become contaminated through contact with the environment and bacterial survival is affected by toothbrush storage containers. [10, 15]

 
 

 
 

TOOTHBRUSH BRISTLES ARE A PERFECT
RESERVOIR FOR BACTERIAL GROWTH AND CHANGING THE TOOTHBRUSH DAILY SHOWED A “SIGNIFICANT REDUCTION” IN ORAL BACTERIA

Bunetel etal. found that bacteria become trapped inside the bristles of the toothbrush [8].Toothbrushes with bristles that are frayed retained more bacteria [15].Glass also found that pathogenic bacteria adhere to plastic after short exposure times [1]. Caudry et al. found that bacteria strongly adhere to the bristles [5]. Mehta et al. found that the retention of moisture and oral debris in the bristles increased bacterial survival [9, 10]. Pai V. concluded “It may be a sound practice to change the toothbrush as frequently as possible” [17]. Sachdev R. et al concluded that there was a “highly significant decrease in oral microorganisms” when comparing use of daily disposable toothbrushes to monthly disposable toothbrushes in the same study subjects [18]. Sachdev R. et al also concluded “As self contaminated multiple-use toothbrushes can aid microorganisms to grow rapidly into the oral cavity, it may be preferable to change the toothbrush as frequently as possible” [18].

Plaque debris buildup on an Interdental Device after just one use.

Used interdental brush. Coloured scanning electron micrograph (SEM) of a bristles from a used interdental brush. They are covered in dental plaque. Plaque consists of a film of bacteria embedded in a glycoprotein matrix. The matrix is formed from bacterial secretions and saliva. Plaque is the main cause of tooth decay. The bacteria feed on sugars in food, producing acid as a waste product. This acid corrodes the teeth's enamel coating, resulting in dental caries. A build-up of dental plaque can also lead to inflamed and infected gums. Severe gum disease can lead to teeth falling out. Magnification: x45 when printed at 10 centimetres wide. Credit: STEVE GSCHMEISSNER / SCIENCE PHOTO LIBRARY

 

DENTAL REFERENCES

 
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BrushPak™ Affiliated Dentists’ dental care recommendations are based on Scientific Medical and Dental Studies***. See BrushPakThe Science for more info and findings found in the following studies.

 
 

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